Jose Group

Group leaders

Title	Name	First Name	Position
Prof. Dr.	Jose	Joachim	Head of the Institute

 For more information on the principal investigator click here...

Researchers

Title	Name	First Name	Position
Dr.	Maas	Ruth	Senior Scientist
Dr.	Sonnenberg	Nadine	Post Doc

BioStruct Fellow

Title	Name	First Name	Position
Dipl.Biol.	Gawarzewski	Iris	PhD student

 For more information on the biostruct fellow click here...

Further Team Members

Technicians:	2
PHD/MD students:	11

First Contact

Joachim Jose
Bioanalytics
Pharmaceutical and Medicinal Chemistry
Heinrich-Heine-University
Universitätsstr. 1, Geb 26.23 02, R40
40225 Düsseldorf
Germany

 +49(0)211 8113848
 +49(0)211 8113847


website:
 http://www.pharmazie.uni-duesseldorf.de/Institute/pharm_chemie/Arbeitskreise/AK_Jose

Second Contact

Ruth Maas
Bioanalytics
Pharmaceutical and Medicinal Chemistry
Heinrich-Heine-University
Universitätsstr. 1, Geb 26.23 02, R40
40225 Düsseldorf
Germany

 +49(0)211 8112525
 +49(0)211 8113847


website:
 http://www.pharmazie.uni-duesseldorf.de/Institute/pharm_chemie/Arbeitskreise/Mitarbeiter/pages/maas

Special Methodical Expertise

• Surface Display of recombinant proteins in E. coli
• Membrane protein expression and preparation
• Biocatalytic synthesis by surface displayed enzymes
• Development of bioanalytic assays

 For detailed information click here...

Main areas of research interest

• Evolutive drug design by surface display library screening
• Biocatalytic synthesis of drug or building blocks by whole cell biocatalysts with surface displayed enzymes
• Test assay development using whole cells of E. coli with surface displayed target proteins

See also:  http://www.pharmazie.uni-duesseldorf.de/Institute/pharm_chemie/Arbeitskreise/AK_Jose/Arbeitsgebiete

Facilities

The laboratories cover the following gene technology security levels:

S1

Laboratory Equipment

General equipment available in the laboratories:

• Biochemistry
• Molecular biology
• Biotechnology
• Microbiology

Special equipment, which is not generally available in most laboratories:

• HPLC-MS, chiral HPLC, capillary electrophoresis, 2D-electrophoresis, elementary analysis, NMR, AAS, microplate fluorescence-, luminescence reader, HPTLC.

Non-standard methods, which are established in the laboratory:

• Surface display library development
• Outer membrane protein isolation

Important references

• Jose J, Jähnig F, Meyer TF (1995) Common structural features of IgA1-protease-like outer membrane protein autotransporters. Mol Microbiol 18: 380-382.
• Jose J, Betscheider D, Zangen D (2005) Bacterial Surface Display Library Screening by target enzyme labelling: Identification of New Human Cathepsin G Inhibitors. Anal Biochem, 346:258-267.
• Jose J, Meyer TF (2007) The autodisplay story – from discovery to biotechnical and biomedical applications. Microbiol Mol Biol R, 71:600-619
• Schultheiss E, Weiss S, Winterer E, Maas R, Heinzle E, Jose J (2008) Esterase autodisplay: enzyme engineering and whole cell activity determination by microplates with integrated pH-sensors. Appl Environ Microbiol, 74:4782-4791.
• Jose J, Chung JW, Jeon JB, Maas RM, Nam CH, Pyun JC (2009) E. coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensensor. Biosens Bioelectron, 24: 1324-1329.

Cooperators

• Pyun, Jae-Chul, Prof. Dr., Yonsei University, Seoul, Korea
• LeBorgne, Marc, Prof. Dr., Université Claude Bernard, Lyon, France
• Olgan, Sureyya, Prof. Dr., Ankara University, Ankara, Turkey
• Bernhardt, Rita, Prof. Dr., Biochemistry, Saarland University, Saarbrücken, Germany
• Meyer Thomas, Prof. Dr., MPI of Infection Biology, Berlin, Germany