Jaeger Group

Group leader

Title	Name	First Name	Position
Prof. Dr.	Jaeger	Karl-Erich	Head of the Institute

 For more information on the principal investigator click here...

Researchers

Title	Name	First Name	Position
Dr.	Krauss	Ulrich	Group leader Molecular Biophotonics
Prof. Dr.	Hummel	Werner	Group leader Biotransformations
Dr.	Drepper	Thomas	Group leader Bacterial Photobiotechnology
Dr.	Rosenau	Frank	Group leader Microbial Expression Systems
Dr.	Wilhelm	Susanne	Group leader Bacterial Autotransporters

BioStruct Fellow

Title	Name	First Name	Position
Msc.	Rani	Raj	PhD student

 For more information on the biostruct fellow click here...

Further Team Members

Technicians:	4
Master/Diploma students:	4
PHD/MD students:	26

First Contact

Institut für Molekulare Enzymtechnologie
Forschungszentrum Jülich
Stetternicher Forst
52426 Jülich
Germany

+49(0)2461-61-3716
+49(0)2461-61-2490


website:
 http://www.iet.uni-duesseldorf.de/

Second Contact

Molecular Biophotonics
Institut für Molekulare Enzymtechnologie
Forschungszentrum Jülich
Stetternicher Forst
52426 Jülich
Germany

+49(0)2461-61-2939
+49(0)2461-61-2490


website:
 http://www.iet.uni-duesseldorf.de/

Special Methodical Expertise

• enzyme gene identification – metagenomics
• protein expression systems
• large-scale (fermenter) protein production and purification
• protein engineering and directed evolution
• photochemistry of photoreceptor proteins

 For detailed information click here...

Main areas of research interest

• Biotechnological applications of photoreceptor proteins
• Structure and function of flavin-binding photoreceptor proteins
• Photobiology and physiology of non-photosynthetic bacteria

Facilities

The laboratories cover the following gene technology security levels:	S1, S2
The laboratories cover the following biohazard levels:	BSL1, BSL2

Laboratory Equipment

General equipment available in the laboratories:

• Biochemistry
• Biotechnology
• Molecular biology
• Microbiology
• Imaging

Special equipment, which is not generally available in most laboratories:

• Colony-picking and liquid-handling robot, automatic bioreactors (up to 15 Liter)

Non-standard methods, which are established in the laboratory:

• Photochemistry of flavo-proteins
• protein-engineering by rational design and by and directed evolution
• high-throughtput screening technology to identify and characterize enzymatic activities and enantioselectivities

Important references

• Drepper, T., T. Eggert, F. Circolone, A. Heck, U. Krauss, J. K. Guterl, M. ,Wendorff, A. Losi, W. Gärtner, and K. E. Jaeger. 2007. Reporter proteins for in vivo fluorescence without oxygen. Nat. Biotechnol 25:443-5.
• Krauss, U., A. Losi, W. Gartner, K. E. Jaeger, and T. Eggert. 2005. Initial characterization of a blue-light sensing, phototropin-related protein from Pseudomonas putida: a paradigm for an extended LOV construct. Phys Chem Chem Phys 7:2804-11.
• Becker, S., H. Hobenreich, A. Vogel, J. Knorr, S. Wilhelm, F. Rosenau, K. E. Jaeger, M. T. Reetz, and H. Kolmar. 2008. Single-cell high-throughput screening to identify enantioselective hydrolytic enzymes. Angew Chem Int Ed Engl 47:5085-8.
• Wilhelm, S., F. Rosenau, S. Becker, S. Buest, S. Hausmann, H. Kolmar, and K. E. Jaeger. 2007. Functional cell-surface display of a lipase-specific chaperone. Chembiochem 8:55-60.
• Tielker D, Eichhof I, Jaeger KE, Ernst JF. 2009 FMN-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell, in press

Cooperators

• Losi, Aba, Dr., Department of Physics-University of Parma, Parma, Italy
• Gärtner, Wolfgang, Prof. Dr., Max-Planck-Institut für Bioanorganische Chemie, Mülheim, Germany
• von Haeseler, Arndt, Prof. Dr., Center for Integrative Bioinformatics, Vienna, Austria
• Benkovic, Stephen J., Prof. Dr., Department of Chemistry, Pennsylvania State University, USA