Hegemann Group

Group leader

Title	Name	First Name	Position
Prof. Dr.	Hegemann	Johannes H.	Head

 For more information on the principal investigator click here...

Researchers

Title	Name	First Name	Position
Dr.	Kerres	Anne	Post Doc
Dr.	Mölleken	Katja	Post Doc

BioStruct Fellow

Title	Name	First Name	Position
M.Sc.	Luczak	Sören	Doctoral researcher

 For more information on the biostruct fellow click here...

Further Team Members

Technicians:	2
PHD/MD students:	8

First Contact

AG Hegemann
Lehrstuhl für Funktionelle Genomforschung der Mikroorganismen
Heinrich-Heine-Universität Düsseldorf
Universitätsstr. 1, Geb. 25.02.U1
40225 Düsseldorf
Germany

 +49(0)211 81-13733
 +49(0)211 81-10637


website:
 http://www.biologie.uni-duesseldorf.de/Institute/Funktionelle_Genomforschung

Second Contact

AG Fleig
Lehrstuhl für Funktionelle Genomforschung der Mikroorganismen
Heinrich-Heine-Universität Düsseldorf
Universitätsstr. 1, Geb. 25.02.U1
40225 Düsseldorf
Germany

 +49(0)211 81-11581
 +49(0)211 81-10637


website:
 http://www.biologie.uni-duesseldorf.de/Institute/Funktionelle_Genomforschung

Special Methodical Expertise

• Protein purification
• Cell analysis + Cell sorting (FACSAria)
• Yeast molecular genetics
• Yeast 2-Hybrid arrays
• Yeast cellsurface display for the analysis of protein interactions

Main areas of research interest

• Genome stability
• Chromosome segregation
• Genome instability + ageing
• Chlamydia biology
• DNA mismatch repair system (MMR) in yeast + humans

Facilities

The laboratories cover the following gene technology security levels:	S1
The laboratories cover the following biohazard levels:	BSL1, BLS2

Laboratory Equipment

General equipment available in the laboratories:

• Biochemistry
• Cell culture
• Molecular biology
• Cell Biology
• Microbiology
• Imaging

Special equipment, which is not generally available in most laboratories:

• FACS Aria
• ÄKTA Prime
• Axiovert Microscope

Non-standard methods, which are established in the laboratory:

• Yeast display system

Important references

• Moelleken K, Hegemann JH.
  The Chlamydia outer membrane protein OmcB is required for adhesion and exhibits biovar-specific differences in glycosaminoglycan binding. Mol Microbiol. 2008 Jan;67(2):403-19
• Guldener U, Koehler GJ, Haussmann C, Bacher A, Kricke J, Becher D, Hegemann JH.
  Characterization of the Saccharomyces cerevisiae Fol1 Protein: Starvation for C1 Carrier Induces Pseudohyphal Growth. Mol Biol Cell. 2004 Aug;15(8):3811-28.
• Fiedler TA, Karpova TS, Fleig U, Young ME, Cooper JA, Hegemann JH.
  The vesicular transport protein Cgp1p/Vps54p/Tcs3p/Luv1p is required for the integrity of the actin cytoskeleton. Mol Genet Genomics. 2002 Oct;268(2):190-205.
• Giaever G, Chu AM, Ni L, Connelly C, Riles L, Veronneau S, Dow S, Lucau-Danila A, Anderson K, Andre B, Arkin AP, Astromoff A, El-Bakkoury M, Bangham R, Benito R, Brachat S, Campanaro S, Curtiss M, Davis K, Deutschbauer A, Entian KD, Flaherty P, Foury F, Garfinkel DJ, Gerstein M, Gotte D, Guldener U, Hegemann JH, Hempel S, Herman Z, Jaramillo DF, Kelly DE, Kelly SL, Kotter P, LaBonte D, Lamb DC, Lan N, Liang H, Liao H, Liu L, Luo C, Lussier M, Mao R, Menard P, Ooi SL, Revuelta JL, Roberts CJ, Rose M, Ross-Macdonald P, Scherens B, Schimmack G, Shafer B, Shoemaker DD, Sookhai-Mahadeo S, Storms RK, Strathern JN, Valle G, Voet M, Volckaert G, Wang CY, Ward TR, Wilhelmy J, Winzeler EA, Yang Y, Yen G, Youngman E, Yu K, Bussey H, Boeke JD, Snyder M, Philippsen P, Davis RW, Johnston M.
  Functional profiling of the Saccharomyces cerevisiae genome. Nature. 2002 Jul 25;418(6896):387-91.
• Guldener U, Heck S, Fielder T, Beinhauer J, Hegemann JH.
  A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res. 1996 Jul 1;24(13):2519-24.

Cooperators

• Prof. Dr. Roger Rank, Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, USA
• Prof. Dr. med. vet. Pospischil Andreas, Institut für Veterinärpathologie, Universität Zürich, Zürich, Switzerland
• Professor Dr. Alan P. Hudson, Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, USA
• Dr. Agathe Subtil, Institut Pasteur, Unité de Biologie des Interactions Cellulaires, Paris, France