Bott / Brocker Group

Group leader

Title	Name	First Name	Position
Prof. Dr.	Bott	Michael	Head of the Institute
Dr.	Brocker	Melanie	Group leader

 For more information on the principal investigator click here...

Researchers

Title	Name	First Name	Position
Dr.	Polen	Tino	Group leader
Dr.	Eggeling	Lothar	Group leader
Dr.	Freudl	Roland	Group leader
Dr.	Bringer-Meyer	Stephanie	Group leader

BioStruct Fellow

Title	Name	First Name	Position
Dipl.Biol.	Schuplezow	Xenia	PhD student

 For more information on the biostruct fellow click here...

Further Team Members

Technicians:	9
Master/Diploma students:	5
PHD/MD students	20

First Contact

Melanie Brocker
Institute of Biotechnology 1
Forschungszentrum Jülich GmbH
Wilhelm-Johnen-Strasse
52428 Jülich
Germany

 +49(0)2461-61-5168
 +49(0)2461-61-2710

website:
 http://www.fz-juelich.de/ibt/ibt-1

Second Contact

Michael Bott
Institute of biotechnology 1
Forschungszentrum Jülich GmbH
Wilhelm-Johnen-Strasse
52428 Jülich
Germany

 +49(0)2461-61-3294
 +49(0)2461-61-2710

website:
 http://www.fz-juelich.de/ibt/ibt-1

Special Methodical Expertise

• Transcriptomics using DNA microarray technology
• Proteomics including MALDI-TOF mass spectrometry
• Metabolic engineering

Main areas of research interest

• Elucidation of regulatory networks and mechanisms in biotechnologically relevant bacteria by studying transcriptional regulators, two-component signal transduction systems and serine/threonine protein kinases.
• Analysis of the energy metabolism in biotechnologically relevant bacteria by studying the respiratory chain and ATP-generating reactions.
• Development of microbial producer strains for amino acids, organic acids and other metabolites of industrial relevance using metabolic engineering

Facilities

The laboratories cover the following gene technology security levels:

S1

Other security laboratories or special facilities:

• radioactive laboratory

Laboratory Equipment

General equipment available in the laboratories:

• Biochemistry
• Molecular biology
• Biotechnology
• Microbiology

Special equipment, which is not generally available in most laboratories:

• 2-D-gel equipment, fluorescence scanner (GenePix scanner for DNA microarrays and Typhoon scanner for proteomics), Spot-Picker, MALDI-TOF/TOF-MS, GC-MS, Biacore, ITC

Non-standard methods, which are established in the laboratory:

• DNA microarray technology, 2D gel electrophoresis, protein identification and characterization by MALDI-TOF-MS, metabolite analysis by GC-MS, interaction studies using Biacore and isothermal titration calorimetry (ITC).

Important references

• Kaspar, S., Perozzo, R., Reinelt, S., Meyer, M., Pfister, K., Scapozza, L., and Bott, M. (1999) The periplasmic domain of the histidine autokinase CitA functions as a highly specific citrate receptor. Mol. Microbiol. 33, 858-872.
• Niebisch, A. and Bott, M. (2003). Purification of a cytochrome bc1-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunit of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1. J. Biol. Chem. 278: 4339-4346.
• Wennerhold, J., Krug, A. and Bott, M. (2005). The AraC-type regulator RipA represses aconitase and other iron proteins from Corynebacterium under iron limitation and is itself repressed by DtxR. J. Biol. Chem. 280, 40500-40508
• Bode, J. G., Schweigart, J. ,Kehrmann, J., Ehlting, C., Schaper, F., Heinrich, P.C. & Häussinger, D. (2003). TNF-alpha induces tyrosine phosphorylation and recruitment of the Src homology protein-tyrosine phosphatase 2 to the gp130 signal-transducing subunit of the IL-6 receptor complex. J Immunol 171,257-266.
• Frunzke, J., Engels, V., Hasenbein, S., Gätgens, C. and Bott, M. (2008). Coordinated regulation of gluconate catabolism and glucose uptake in Corynebacterium glutamicum by two functionally equivalent transcriptional regulators, GntR1 and GntR2. Mol. Microbiol. 67, 305-322

Cooperators

• Eikmanns, Bernd, Prof. Dr., University of Ulm, Germany
• Bramkamp, Marc, Dr., University of Cologne, Germany
• Wendisch, Volker, Prof. Dr., University of Münster, Germany
• Burkovski, Andreas, Prof. Dr., University of Erlangen-Nürnberg, Germany
• Pohl, Ehmke, Dr., University of Durham, U.K.