Schmitt Group

Group leader

Title	Name	First Name	Position
Prof. Dr.	Schmitt	Lutz	Head of the Institute

 For more information on the principal investigator click here...

Researchers

Title	Name	First Name	Position
Dr.	Schulte	Ulrich	Senior scientist
Dr.	Hanekop	Nils	PostDoc
Dr.	Smits	Sander	Senior scientist
Dr.	Patrik	Bakkes	Post Doc

BioStruct Fellow

Title	Name	First Name	Position
Dipl.-Biol.	Meyer	Tatu	PhD student

 For more information on the biostruct fellow click here...

Further Team Members

Technicians:	3
Master/Diploma students:	6
PHD/MD students:	10

Contact

Lutz Schmitt
Institute of Biochemistry
Heinrich Heine University
Universitaetsstr. 1
40225 Duesseldorf

+49(0)211-81-10773
+49(0)211-81-15310


website:
http://www.uni-duesseldorf.de/WWW/MathNat/biochem/schmitt/Startpage.htm

Contact 2

Sander Smits

+49(0)211-81-12647
+49(0)211-81-15310

Expertise in Structural Biology

• X-ray crystallography
• Fluorescence Spectroscopy

 click here for further information

Main areas of research interest

• Structure and function of ABC-transporters
• Transport processes across biological membranes
• Protein-protein interaction
• Protein structure
• Recognition processes at membranes

Facilities

The laboratories cover the following gene technology security levels:

S1

Other security laboratories or special facilities:

• Radioactive laboratory

Laboratory Equipment

General equipment available in the laboratories:

• Biochemistry
• Biophysics
• Molecular biology
• Microbiology

Special equipment, which is not generally available in most laboratories:

• X-ray Generator
• Crystallization Robot
• ITC Setup

Non-standard methods, which are established in the laboratory

• In vitro translation
• Incorporation of non-natural amino acids

Important References

• L. Schmitt, H. Benabdelhak, M. A. Blight, I. B. Holland & M. T. Stubbs (2003) Crystal structure of the NBD of the ABC-transporter HlyB: Identification of a variable region within the ABC helical domains, J. Mol. Biol. 330 333-343.
• J. Zaitseva, S. Jenewein, T. Jumpertz, I. B. Holland, & L. Schmitt (2005) H662 is the linchpin in ATP hydrolysis of the nucleotide-binding domain of the ABC-transporter HlyB, EMBO J. 24 1901-1910.
• J. Zaitseva, C. Oswald, T. Jumpertz, S. Jenewein, A. Wiedenmann, I. B. Holland & L. Schmitt (2006) A structural analysis of asymmetry required for catalytic activity in an isolated ABC-ATPase domain dimer. EMBO J. 25 3432- 3443.
• C. Horn, S. Jenewein, B. Tschapek, W. Bouschen, S. Metzger, E. Bremer & L. Schmitt (2008) Monitoring conformational changes during the catalytic cycle of OpuAA, the ATPase subunit of the ABC-transporter OpuA from Bacillus subtilis, Biochem. J., 412 233- 244.
• R. Ernst, P. Kueppers, C. Klein, T. Schwarzmueller, K. Kuchler & L. Schmitt (2008) A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5 Proc. Natl. Acad. Sci USA 105 5069-5074.

Cooperators

• Holland, Barry, Prof. Dr., University of Paris-Sud, Orsay, France
• Kuchler, Karl, Prof. Dr., Medical University of Vienna, Austria
• Dötsch, Volker, Prof. Dr., University of Frankfurt, Germany
• Driessen, Arnold, Prof. Dr., University of Groningen, Netherlands
• Volkmer, Andreas, Dr., University of Stuttgart, Germany