Ms. Sabine Eisenbürger

Bode Group Institute for Gastroenterology, Hepatology and Infectiology Heinrich Heine University Universitässtr.1, Geb. 23.12 U1. 65 40225 Düsseldorf, Germany  +49(0)211-81-15639

Mini Academic CV

University degrees:

First degree or intermediate examination:

• state examination pharmacy, 2007, Heinrich-Heine University, D�sseldorf, Germany

Second degree and/or intermediate examination:

• state examination pharmacy, 2009, Heinrich-Heine University, D�sseldorf, Germany

BioStruct PhD project

Analysis of the structure of the molecular interaction between HCV NS5B polymerase and c-Src
The hepatitis C virus (HCV) is worldwide a major cause of chronic liver disease with a high tendency to establish a persistent infection. To replicate in its host cell the virus has evolved mechanisms to utilize and control cellular molecules or pathways required for virus genome replication. We could demonstrate that functional c-Src is required for sufficient replication of HCV (unpublished data). A more detailed analysis of the interaction of c-Src with HCV encoded proteins by immunoprecipitation studies or by pull-down experiments using GST-tagged proteins revealed that c-Src physically interacts with the RNA-dependent RNA polymerase NS5B of HCV. In particular the isolated SH3 domain of c-Src strongly interacts with NS5B. Further studies suggested that in part also the SH2 but not the SH1 domain of c-Src interacts with NS5B (unpublished data). We are currently focusing on the exact localization of the regions of NS5B required for this interaction and on the evaluation of the differential contributions of the SH2 and SH3 domain of c-Src to the interaction with NS5B. Based on these data, the aim of the project is to resolve the structure of the interaction of the isolated SH3 (and/or SH2) domain of c-Src with the respective region of NS5B by NMR studies. For this purpose GST-fusion proteins comprising the respective domains of c-Src and NS5B will be expressed and purified. A second part of the project aims to analyze the structure of the complex of the whole proteins of c-Src and NS5B using X-ray analysis.


Topic Supervisor: PD Dr. Johannes Bode, Department of Gastroenterology, Hepatology and Infectiology, Bode Group

Complementary Supervisor: Prof. Dr. Dieter Willbold, Institute for Plant Biochemistry, Heinrich Heine University Duesseldorf, Willbold Group

BioStruct Fellow: Ms. Sabine Eisenbürger