The Cord Blood Bank at the Bone Marrow Donor Center Düsseldorf is the German Branch of the European Cord Blood Bank (ECBB-GER).

Contact

• Operating Director Gesine Kögler, Ph.D.: Phone: +49-211-81-18684; Fax: +49-211-81 16 792
• Koegler@itz.uni-duesseldorf.de

Information available on frozen cord blood samples

1. HLA Class I and II typing of the cord blood:
   • HLA-A, B, C typing by serology; one stored sample for confirmatory HLA typing available
   • HLA-DR, DQ typing by PCR-SSO; generic typing of HLA-DRß, -DQß
   • subtyping of HLA-DRß, -DQß and -DPß upon request
2. Nucleated cell count and CD34 positive cells
3. Blood group: ABO, D, Kell, Duffy of the cord blood
4. Infectious disease testing
   • of the mother:
     • HIV1/2; HTLV-1/2; Hepatitis A, B, C; Toxoplasmosis; Syphilis;
     • CMV antibody, total and IgM; HSV, IgG and IgM; EBV, IgG and IgM
   • of the cord blood: CMV (PCR)
5. Control of sterility: aerobic and anaerobic cultures
6. Number of hematopoietic precursor cells: CFU-GM, BFU-E, CFU-GEMM
7. Stored specimen of the cord blood and the maternal blood: DNA, mononuclear cells Plasma/serum of the mother
8. Testing for the exclusion of contaminating maternal cells
   • X/Y chromosome analysis in sexmismatched situations
   • PCR-SSP for the noninherited maternal HLA-DRß allele
9. Information available of the mother's interview and review of diseases on the mother and the child at discharge.
   • Diseases (genetic and infections)
   • Problems with pregnancy, delivery and after birth
   • Ethnicity of the parents