5. Sustainable Agriculture, Fisheries
and Forestry
SIDS
CONTRACT NUMBER: QLRT-1999-01567
TITLE:
SEXUAL IDENTIFICATION
AND DEVELOPMENT IN THE SWORDFISH - IMPROVED DETERMINATION TOOLS
FOR MORE EFFICIENT STOCK ASSESSMENT AND IMPLEMENTATION OF CONTROL
MEASURES
GENERAL INFO: TYPE: RS TOTAL COST: 385.422 Euro:
DURATION: 32 AREA: 5.1.2 TOTAL EC CONT: 344.465 START DATE: MARCH
2000
ABSTRACT:
IN THE ECONOMICALLY
IMPORTANT MEDITERRANEAN SWORDFISH FISHERY THE DETERMINATION OF SEX AND GONADAL
MATURITY AT CAPTURE ARE IMPORTANT REQUISITES FOR REGULATION. THESE
PARAMETERS CANNOT ALWAYS BE RESOLVED DUE TO "GUTTING AT SEA", LACK OF
EXTERNAL SEXUAL DIMORPHISM OR HIGH MARKET MUSCLE VALUE PRECLUDING
GUTTING. THE OBJECTIVE OF THIS STUDY IS TO DEVELOP A MORE EFFICIENT, COST
EFFECTIVE ASSESSMENT METHOD FOR SEX AND GONADAL MATURITY USING AN ELISA
MEDIATED MUSCLE BIOPSY ASSAY TO MEASURE THE LEVELS OF SEX HORMONES AND THE
LIPOPROTEIN VITELLOGENIN FROM SWORDFISH MUSCLE SAMPLES. THESE SUBSTANCES CAN BE
USED AS INDICATORS OF SEX AND SPAWNING STATE OF THE FISH AND CORRELATED WITH
GONADAL DEVELOPMENT. THE NEW MUSCLE BIOPSY METHOD WILL BE RELEVANT FOR
OTHER MARINE SPECIES PROVIDING A VALUABLE MANAGEMENT TOOL TO OVERSEE
CONSERVATION MEASURES IN THE MARKET PLACE IN TERMS OF SPECIES, SEX AND GONDAL
MATURITY FROM SINGLE MUSCLE SAMPLES.
CO-ORDINATOR:
DR. C. BRIDGES
HEINRICH-HEINE UNIVERSITY DUESSELDORF
UNIVERSITÄTSSTRASSE 1
D - 40225 DÜSSELDORF CEDEX
TEL: +49-2118114991
FAX: +49-2118114991
E-MAIL: bridges@uni-duesseldorf.de
PARTNERS:
DR. D. KIME
DEPARTMENT OF ANIMAL AND
PLANT SCIENCES
WESTERN BANK, ALFRED DENNY
BUILDING
UNIVERSITÄTSSTRASSE 1 UK -
S10 2TN SHEFFIELD
TEL: +44-1142224316
FAX: +44-1142220002
E-MAIL:
d.kime@sheffield.ac.uk
DR. M. APOSTOLOPOULOU & DR P. MEGALOFONOU
NATIONAL AND KAPODISTRIAN
UNIVERSITY OF ATHENS
DEPARTMENT OF ZOOLOGY -
MARINE BIOLOGY FACULTY OF
PANEPISTIMIOPOLI
EL - 157 84 ATHENS
E-MAIL:
yannmega@biology.db.uoa.gr
TEL: +30-17233634
FAX: +30-17274604
DR. JOSE MIGUEL DE LA SERNA
CENTRO OCEANOGRÁFICO DE
MALAGA -INSTITUTO ESPAÑOL DE OCEANOGRAFÍA
S, ILISIA MUELLE PESQUERO
S/N // P.O. BOX 285
E - 29640 MALAGA
TEL: +34-52472261
FAX: +34-52463808
E-MAIL:
delaserna@ma.ieo.es
DR. G. DE METRIO
UNIVERSITY OF BARI
DEPARTMENT OF ANIMAL PRODUCTION
DEPARTMENT OF ANIMAL
PRODUCTION
VIA AMENDOLA 165/A
I - 70126 BARI
TEL: +39-804670604
FAX: +39-804670283
E-MAIL: G.Demetrio@tno.it
OBJECTIVES
a) Identification and isolation of sex hormones and vitellogenin in the plasma and
muscle biopsy samples taken from the swordfish.
b) Development of an ELISA-mediated standard test tools to determine the
concentrations the above substances in both plasma and muscle.
c) Verification of the correlation between hormone and vitellogenin levels in
both plasma and muscle biopsy samples with biometric data on gonadal indices
and sex ratio.
d)Test and evaluate muscle biopsy in both field (double blind) and market
conditions.
e) Provide a standard muscle biopsy method for fisheries stock assessment of
sex ratio and gonadal maturity for marine fisheries of the Mediterranean .
First SIDS coordination meeting in Düsseldorf 2- 4- March 2000:
Upper row from left to
right: Aldo Correia (Bari) Gregorio Demetrio (Bari), Heinrich Heine
(Düsseldorf), David Kime (Sheffield), Persefoni Megalofanou (Athens) Miguel de
La Serna
(Malaga), ; Lower Row Jurg Eicker (Düsseldorf), Rosario
Paciello (Düsseldorf), Vito
Susca (Düsseldorf) and Chris Bridges (Düsseldorf)
SWORDFISH Stabiliy
Protocol Landing with Franco´s Boat from La Scogliera
NEWS!!!!!!!
1.
INTERIM REPORT ON STABILITY OF SAMPLES
Exploitation
and Dissemination Activities
Abstracts
are available
Publications
NEW !!!! Preprint: Endocrine Disruption Paper.
NEW !!!! NEW SCIENTIST REPORT
V. Susca, A. Corriero M. Deflorio, C.R. Bridges, G.
De Metrio (2000) New results on the reproductive
biology of the bluefin tuna (thunnus
thynnus) in the Mediterranean. ICCAT Scientific Report : SCRS /00/191
C.R. Bridges, P. Schröder ,V. Susca A Corriero, M. Deflorio and Gregorio
De Metrio (2000)
A New Muscle Biopsy Technique for Sex and Sexual Maturity
Determination in Large Pelagic Fishes
ICCAT
Scientific Report SCRS/2000/192
V. Susca, A. Corriero, C.R. Bridges and G. De
Metrio. 2001. Study of the sexual maturity of fenmale bluefin tuna:
purification and partial characterization of vitellogenin and
its use in an enzyme-linked Immunosorbent assay
Journal of Fish Biology 58, 815-831
Presentations
A New Muscle Biopsy Technique for Sex and Sexual
Maturity Determination in Large Pelagic Fishes.
Bridges, C.R., Susca, V., A. Corriero & De Metrio, G
Eurocean Conference 2000 Sept. Hamburg
A11.1 Fishy Business in the
Meditterranean - Tuna, Tonnara and Testosterone
C. R. Bridges and V. Susca and J.
Eicker (Düsseldorf); A. Corriero and G. De Metrio ( Bari) SEB Meeting
Canterbury March 2001.
“Fishy business in the
meditteranean - tuna, tonnara and testosteron?”
C.R. Bridges,V. Susca, J. Eicker, A. Corriero, G. De Metrio,
P. Megalofonou, M. de la Serna, D.Kime.
Seminar
:Rosenstiel School of Marine and Atmospheric Science . Univ. Miami and NOAA,
Miami. February 2001
European
Perspectives – Examples from CFP and FP5 Programmes and Lessons learned for FP6
C.R. Bridges,V. Susca, J. Eicker, A. Corriero, G. De Metrio,
P. Megalofonou, M. de la Serna, D.Kime.
DEMA meeting Liverpool April 2001
Posters:
A11.14 Vitellogenin of the
Mediterranean Swordfish (Xiphias gladius)- Purification, partial
characterisation and measurement of plasma levels.
J.Eicker, V.Susca, C.R.Bridges, D, Kime, M. de La Serna, P. Megalofonou, A.
Corriero; S.and G. De Metrio . SEB Canterbury March 2001
A11. 15 Determination of First
Sexual Maturity in Mediterranean Bluefin Tuna (Thunnus Thynnus)
V. Susca, C.R. Bridges, A. Corriero; S.and G. De Metrio. SEB Canterbury March 2001