5. Sustainable Agriculture, Fisheries and Forestry

SIDS

CONTRACT NUMBER:  QLRT-1999-01567

TITLE:
SEXUAL IDENTIFICATION AND DEVELOPMENT IN THE SWORDFISH - IMPROVED DETERMINATION TOOLS FOR MORE EFFICIENT STOCK ASSESSMENT AND IMPLEMENTATION OF CONTROL MEASURES

GENERAL INFO: TYPE: RS TOTAL COST: 385.422 Euro: DURATION: 32  AREA: 5.1.2 TOTAL EC CONT: 344.465 START DATE: MARCH 2000

 ABSTRACT:
IN THE ECONOMICALLY IMPORTANT MEDITERRANEAN SWORDFISH FISHERY THE DETERMINATION OF SEX AND GONADAL MATURITY AT CAPTURE ARE IMPORTANT REQUISITES FOR REGULATION.  THESE PARAMETERS CANNOT ALWAYS BE RESOLVED DUE TO "GUTTING AT SEA", LACK OF EXTERNAL SEXUAL DIMORPHISM OR HIGH MARKET MUSCLE VALUE PRECLUDING GUTTING.  THE OBJECTIVE OF THIS STUDY IS TO DEVELOP A MORE EFFICIENT, COST EFFECTIVE ASSESSMENT METHOD FOR SEX AND GONADAL MATURITY USING AN ELISA MEDIATED MUSCLE BIOPSY ASSAY TO MEASURE THE LEVELS OF SEX HORMONES AND THE LIPOPROTEIN VITELLOGENIN FROM SWORDFISH MUSCLE SAMPLES. THESE SUBSTANCES CAN BE USED AS INDICATORS OF SEX AND SPAWNING STATE OF THE FISH AND CORRELATED WITH GONADAL DEVELOPMENT.  THE NEW MUSCLE BIOPSY METHOD WILL BE RELEVANT FOR OTHER MARINE SPECIES PROVIDING A VALUABLE MANAGEMENT TOOL TO OVERSEE CONSERVATION MEASURES IN THE MARKET PLACE IN TERMS OF SPECIES, SEX AND GONDAL MATURITY FROM SINGLE MUSCLE SAMPLES.

CO-ORDINATOR:
DR. C. BRIDGES
HEINRICH-HEINE UNIVERSITY DUESSELDORF
UNIVERSITÄTSSTRASSE  1
D - 40225 DÜSSELDORF CEDEX
TEL: +49-2118114991
FAX: +49-2118114991
E-MAIL: bridges@uni-duesseldorf.de

PARTNERS:
DR. D. KIME
DEPARTMENT OF ANIMAL AND PLANT SCIENCES
WESTERN BANK, ALFRED DENNY BUILDING
UNIVERSITÄTSSTRASSE 1 UK - S10 2TN SHEFFIELD
TEL: +44-1142224316
FAX: +44-1142220002
E-MAIL: d.kime@sheffield.ac.uk

DR. M. APOSTOLOPOULOU &  DR P. MEGALOFONOU
NATIONAL AND KAPODISTRIAN UNIVERSITY OF ATHENS
DEPARTMENT OF ZOOLOGY - MARINE BIOLOGY FACULTY OF
PANEPISTIMIOPOLI
EL - 157 84 ATHENS
E-MAIL: yannmega@biology.db.uoa.gr
TEL: +30-17233634
FAX: +30-17274604

DR. JOSE MIGUEL DE LA SERNA
CENTRO OCEANOGRÁFICO DE MALAGA -INSTITUTO ESPAÑOL DE OCEANOGRAFÍA
S, ILISIA MUELLE PESQUERO S/N // P.O. BOX 285
 E - 29640 MALAGA
TEL: +34-52472261
 FAX: +34-52463808
 E-MAIL: delaserna@ma.ieo.es

DR. G.  DE METRIO
UNIVERSITY OF BARI DEPARTMENT OF ANIMAL PRODUCTION
DEPARTMENT OF ANIMAL PRODUCTION
VIA AMENDOLA 165/A
I - 70126 BARI
TEL: +39-804670604
FAX: +39-804670283
E-MAIL: G.Demetrio@tno.it

OBJECTIVES
a) Identification and isolation of sex hormones and vitellogenin in the plasma and muscle biopsy samples taken from the swordfish.
b) Development of an ELISA-mediated standard test tools to determine the concentrations the above substances in both plasma and muscle.
c) Verification of the correlation between hormone and vitellogenin levels in both plasma and muscle biopsy samples with biometric data on gonadal indices and sex ratio.
d)Test and evaluate muscle biopsy in both field (double blind) and market conditions.
e) Provide a standard muscle biopsy method for fisheries stock assessment of sex ratio and gonadal maturity for marine fisheries of the Mediterranean .

First SIDS coordination meeting in Düsseldorf 2- 4- March 2000:

Upper row from  left to right: Aldo Correia (Bari) Gregorio Demetrio (Bari), Heinrich Heine
(Düsseldorf), David Kime (Sheffield), Persefoni Megalofanou (Athens) Miguel de La Serna
(Malaga),  ; Lower Row  Jurg  Eicker (Düsseldorf), Rosario Paciello (Düsseldorf), Vito
Susca (Düsseldorf) and Chris Bridges (Düsseldorf)

SWORDFISH Stabiliy Protocol Landing with Franco´s Boat from La Scogliera

 

 NEWS!!!!!!!

1.      INTERIM REPORT ON STABILITY OF SAMPLES

2.      FIRST PROGRESS REPORT

3.      SECOND PROGRESS REPORT

4.      FINAL REPORT NEW !!!!

Exploitation and Dissemination Activities

 

Abstracts are available

 

Publications

 

NEW !!!! Preprint: Endocrine Disruption Paper.

 

NEW !!!! NEW SCIENTIST REPORT

V. Susca, A. Corriero M. Deflorio, C.R. Bridges, G. De Metrio (2000) New results on the reproductive biology of the bluefin tuna (thunnus thynnus) in the Mediterranean. ICCAT Scientific Report : SCRS /00/191

 

C.R. Bridges, P. Schröder ,V. Susca A Corriero, M. Deflorio and Gregorio De Metrio (2000)

A New Muscle Biopsy Technique for Sex and Sexual Maturity Determination in Large Pelagic Fishes

 ICCAT Scientific Report SCRS/2000/192

 

V. Susca, A. Corriero, C.R. Bridges and G. De Metrio. 2001. Study of the sexual maturity of fenmale bluefin tuna:

 purification and partial characterization of vitellogenin and its use in an enzyme-linked Immunosorbent assay

 Journal of Fish Biology 58, 815-831

 

 

Presentations

A New Muscle Biopsy Technique for Sex and Sexual Maturity Determination in Large Pelagic Fishes.

Bridges, C.R., Susca, V., A. Corriero &  De Metrio, G

Eurocean  Conference 2000 Sept. Hamburg

 

A11.1 Fishy Business in the Meditterranean - Tuna, Tonnara and Testosterone

C. R. Bridges and V. Susca and J. Eicker (Düsseldorf); A. Corriero and G. De Metrio ( Bari) SEB Meeting Canterbury March 2001.

 

“Fishy business in the  meditteranean - tuna, tonnara and testosteron?”

C.R. Bridges,V. Susca, J. Eicker, A. Corriero, G. De Metrio, P. Megalofonou, M. de la Serna, D.Kime.

 Seminar :Rosenstiel School of Marine and Atmospheric Science . Univ. Miami and NOAA, Miami. February 2001

 

European Perspectives – Examples from CFP and FP5 Programmes and Lessons learned for FP6

C.R. Bridges,V. Susca, J. Eicker, A. Corriero, G. De Metrio, P. Megalofonou, M. de la Serna, D.Kime.

DEMA meeting Liverpool April 2001

 

Posters:

A11.14 Vitellogenin of the Mediterranean Swordfish (Xiphias gladius)- Purification, partial characterisation and measurement of plasma levels.

J.Eicker, V.Susca, C.R.Bridges, D, Kime,  M. de La Serna, P. Megalofonou, A. Corriero; S.and G. De Metrio . SEB Canterbury  March 2001

 

A11. 15 Determination of First Sexual Maturity in Mediterranean Bluefin Tuna (Thunnus Thynnus)

V. Susca, C.R. Bridges,  A. Corriero; S.and G. De Metrio. SEB Canterbury March 2001