Genetic Parasitology

Research Group Prof. Dr. Werner Kunz

Institute for Genetics

Heinrich-Heine-University Duesseldorf

Universitätsstr.1 Geb.: 26.03.U1

D-40225 Düsseldorf Germany



 
 


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Project description

Research topic of the entire group is sex-specific gene expression in Schistosoma mansoni. In the main focus are those genes in females and males that are controlled by pairing of the two genders. The male induces the expression of a couple of genes in the female that are involved in egg formation. Having established an in-vitro system to study female gene expression in the presence or absence of the male, we now focus on signal transducing molecules that transfer the male inductive signal to proliferating germinative cells in the female reproductive organs. Such molecules contain highly conserved domains which opened the way to PCR amplification experiments as well as to diverse screening procedures with heterologous probes. The first signal transducing molecules in schistosomes have recently been identified in our lab that are gender-specifically and pairing-dependently expressed and may thus be involved in the induction of female fertility by the male. 

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Our research group:

Prof. Dr. Werner Kunz
Tel.: (49)-211-81-12333
Fax: (49)-211-81-12333
email: werner.kunz@uni-duesseldorf.de


Dr. Christoph G. Grevelding
Tel.: (49)-211-81-13070
Fax: (49)-211-81-12333
email: christoph.grevelding@uni-duesseldorf.de


research interest: male-specific and pairing-dependent gene expression, male-female interaction, signal transduction,                                   transformation, sex chromosoms

Andreas Kampkötter
Tel.: (49)-211-81-12077
Fax: (49)-211-81-12333
email: andreas.kampkoetter@uni-duesseldorf.de


research interest: RNA display, differential gene expression, signal transduction, sexing of schistosomes

Ralf Winnen
Tel.: (49)-211-81-12077
Fax: (49)-211-81-12333
email: ralf.winnen@uni-duesseldorf.de


research interest: signal transduction, protein of tegument, achetylcholinesterases, phospholipases

Christian Kutzick
Tel.: (49)-211-81-14808
Fax: (49)-211-81-12333
email: kutzick@mail.rz.uni-duesseldorf.de


research interest: signal transduction (Rap), differential hybridisation, construction of cDNA-libraries

Katja Kapp
Tel.: (49)-211-81-12075
Fax: (49)-211-81-12333
email: katja.kapp@uni-duesseldorf.de


research interest: signal transduction, SH2 proteins, transformation of schistosomes

Mirko Bier
Tel.: (49)-211-81-12075
Fax: (49)-211-81-12333
email: bier@uni-duesseldorf.de


research interest: signal transduction, SH2 proteins, in vitro culture

Albert Kaltenberg
Tel.: (49)-211-81-14808
Fax: (49)-211-81-12333
email: kaltenb@uni-duesseldorf.de


research interest: signal transduction, mitogen activated phosphatases (MAP), in vitro culture

Jürgen Knobloch

Tel.: (49)-211-81-12408

Fax: (49)-211-81-2333

email: knobloch@uni-duesseldorf.de

research interest: signal transduction, Receptortyrosin kinases, RNA-gelelution

Stefanie Sudhop

Tel.: (49)-211-81-12408

Fax: (49)-211-81-2333

email: sudhop@uni-duesseldorf.de

research interest: differential gene expression, RNA-fingerprints, male-female interaction

Karin Opatz
Tel.: (49)-211-81-12407
Fax: (49)-211-81-12333


research interest: chromosome preparation, maintenance of recombinant clones

Didina David
Tel.: (49)-211-81-13070
Fax: (49)-211-81-12333


research interest: culture of schistosomes, intermediate and finale host infection 


some actual papers of our group:

Finken-Eigen, M., Kunz, W.Schistosoma mansoni: Gene structure and localization of the cysteine protease ER 60. Exp. Parasitol., 86: 1-7. 1997

Grevelding, C.G., Kampkötter, A., and Kunz, W. Schistosoma mansoni: PCR on adult and larval stages without DNA extraction. Exp. Parasitol. 85: 99-100, 1997.

Schüßler, P., Grevelding, C.G., and Kunz, W. Cloning and characterization of elongation factor 1-alpha of Schistosoma mansoni. 83: 206-208, 1997.

Grevelding, C.G., Sommer, G., and Kunz, W. Female-specific gene expression in Schistosoma mansoni regulated by pairing. Parasitology, in press.

Schüßler, P., Grevelding, C.G., and Kunz, W. Identification of Ras, MAP kinases, and a GAP protein in Schistosoma mansoni by immunoblotting and their putative involvement in male-female interaction. Parasitology, in press.

Kampkötter, A., Ridgers, I., Johnston, D.A., Rollinson, D., Kunz, W., Grevelding, C.G. Cloning and characterization of the ras-homologue in Schistosoma mansoni. submitted.

Sommer, G., Davis, R. E., Maniak, M., Kunz, W. Expression of an antigen recognized by sera from infected humans in the oesophageal glands of Schistosoma mansoni. submitted.

Schüßler, P., Köhrer, K., Finken-Eigen, M., Grevelding, C.G., and Kunz, W. A female-specific cDNA sequence of Schistosoma mansoni encoding an amidase that is expressed in the gastrodermis. submitted.

Klinkert, M.-Q. and Kunz, W. Digestion of haemoglobin by schistosomes. Parasitol. Today 12: 165, 1996.

Schmidt, J., Bodor, O., Gohr, L., Kunz, W. Paramyosin isoforms of Schistosoma mansoni are phosphorylated and localized in a large variety of muscle types. Parasitology 112:459-467, 1996.

Schüßler, P., Grevelding, C.G., and Kunz, W. More WWW addresses for parasitologists. Parasitol. Today 12: 291, 1996.

Schüßler, P., Pötters, E., Winnen, R., Michel, A., Bottke, W., and Kunz, W. Ferritin mRNAs in Schistosoma mansoni do not have iron-responsive elements for post-transcriptional regulation. Eur. J. Biochem., 241: 64-69, 1996.

Grevelding, C.G., Kampkötter, A., Hollmann, M., Schaefer, U., Kunz, W. Direct PCR on fruitflies and blood flukes without prior DNA isolation. Nucleic Acids Res. 24(20): 4100-4101, 1996.

Butler, R., Michel, A., Kunz, W., Klinkert, M.-Q. Sequence of Schistosoma mansoni cathepsin C and its structural comparison with papain and cathepsins B and L of the parasite. Prot. Pept. Letters, 2: 313-320, 1995.

Grevelding, C.G. The female-specific W1 sequence of the Puerto Rican strain of Schistosoma mansoni occurs in both genders of a Liberian strain. Mol. Biochem. Parasitol. 71: 269-272, 1995.

Kunz, W., Gohr, L.G., Grevelding, C.G., Schüßler, P., Sommer, G., Menrath, M., and Michel, A. Schistosoma mansoni: Control of female fertility by the male. Mem. Inst. Oswaldo Cruz 90(2): 185-189, 1995.

Kunz,W., Grevelding, C.G., Gohr, L. Schistosome research newsgroup. Parasitol. Res. 81: A3, 1995.

Menrath,M., Michel, A., Kunz, W. A female-specific cDNA sequence of Schistosoma mansoni encoding a mucein-like protein that is expressed in the epithelial cells of the reproductive duct. Parasitology 111: 477-483, 1995.

Michel, A., Ghoneim, H., Resto, M., Klinkert, M.-Q., Kunz, W. Sequence, characterization and lokalization of a cysteine proteinase cathepsin L in Schistosoma mansoni Mol. Biochem. Parasitol. 73: 7-18, 1995.

Schüßler, P., Pötters, E., Winnen, R., Bottke, W., and Kunz, W. An isoform of ferritin as a component of protein yolk platelets in Schistosoma mansoni. Mol. Reprod. Dev. 41: 325-330, 1995.

Schüßler, P., Gohr, L.G., Sommer, G., Kunz, W., and Grevelding, C.G. A simple method for the combined isolation of nucleic acids and protein from minor amounts ot tissue. Trends Genet. 11(10): 378-379, 1995.

Finken, M., Sobek, A., Symmons, P., and Kunz, W. Characterization of the complete protein disulfide isomerase gene of Schistosoma mansoni and identification of the tissues of its expression. Mol. Biochem. Parasitol. 64:135-144, 1994.

Weston, D., Schmitz, J., Kemp, W.M., and Kunz, W. Cloning and sequence of a complete myosin heavy chain cDNA from Schistosoma mansoni.Mol. Biochem. Parasitol. 58:161-164, 1993.

Dietzel, J., Hirzmann, J., Preis, D., Symmons, P., and Kunz, W. Ferritins of Schistosoma mansoni: Sequence comparison and expression in female and male worms. Mol. Biochem. Parasitol. 50:245-254, 1992.

Höltke, H.J., Ettl, I., Finken, M., West, S., and Kunz, W. Multiple nucleic acid labeling and rainbow detection. Anal. Biochem. 207:24-31, 1992.

Kunz, W. and West, S. Azo dyes. In: Nonradioactive labeling and detection of biomolecules, edited by Kessler, C.Berlin:Springer-Verlag, 1992,p. 161-164.

Schröder, J. , Kunz, W. Monospecific antibodies: Affinity purificatio by filter bound recombinant antigens. Bioforum 7-8, 235-239, 1991.

West, S. , Schröder, J., Kunz, W. A multple-staining procedure for the detection of different DNA fragments on a single blot. Anal. Biochem. 190, 254-258, 1990.

Schröder, J., Symmons, P., and Kunz, W. Immunenzymatische Farbkodierung zur Erkennung von DNA-Klonen in Expressionsvektor-Bibliotheken. BioEng. 5:24-30, 1989.

Köster, B., Dargatz, H., Schröder, J., Hirzmann, J., Haarmann, C., Symmons, P., and Kunz, W. Identification and localisation of the products of a putative eggshell precursor gene in the vitellarium of Schistosoma mansoni. Mol. Biochem. Parasitol. 31:183-198, 1988.

Kunz, W. Genetics of parasites. In: Parasitology in focus: facts and trends, edited by H. Mehlhorn, Berlin:Springer-Verlag, p. 368-390, 1988.

Kunz, W., Opatz, K., Finken, M., and Symmons, P. Sequences of two genomic fragments containing an identical coding region for a putative eggshell precursor protein of Schistosoma mansoni. Nucleic Acids Res.15:5894, 1987.

Kunz,W., Symmons, P. Gender-specifically expressed genes in Schistosoma mansoni. Acta Trop. 44:90-93, 1987.

Meusel, G., Symmons, P., and Kunz, W. Two gender-specific transcripts predominate in the mRNA of adult female Schistosoma mansoni. J.Parasitol. 73:436-437, 1987. 


Andreas Kampkötter - Genetic Parasitology
E-Mail: andreas.kampkoetter@.uni-duesseldorf.de

July 1998

This page is still under construction. Any suggestions and criticisms are welcome.

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